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Image Search Results
Journal: Oncotarget
Article Title: Hedgehog signaling sensitizes Glioma stem cells to endogenous nano-irradiation
doi:
Figure Lengend Snippet: (A) Representative pictures of CD133 (green staining) and GFAP (green staining) immunofluorescence staining in GBM cells R8, R18 and R28 (Hoechst nuclei staining in blue). (B) mRNA expression levels of Ptch1, Smo and Gli1 in R8, R18 and R28 GBM cells. Actin-β served as a loading control. (C) SDS-PAGE and Western blot analysis of HH pathway components Ptch1, Smo and Gli1 in separated CD133 − and CD133 + subpopulations of R28 cells. GAPDH served as a loading control.
Article Snippet: For isolation of CD133 positive stem cells, the
Techniques: Staining, Immunofluorescence, Expressing, SDS Page, Western Blot
Journal: Oncotarget
Article Title: Hedgehog signaling sensitizes Glioma stem cells to endogenous nano-irradiation
doi:
Figure Lengend Snippet: (A) Percentage of BrdU + R8, R18, R28 cells and normal human astrocytes (NHA) after stimulation with SHH in comparison with unstimulated cells. Data are mean ± SD from three experiments. (B) Phase-contrast images (i, iv) and fluorescent images after staining with BODIPY-Cyclopamine (anti-Smo, green; ii, v) and anti-BrdU antibody (green; iii, vi) of unstimulated and SHH stimulated R28 neurospheres (Hoechst nuclei staining in blue). (C) Representative cytometries of anti-BrdU-FITC and 7-AAD (total DNA) double staining of unstimulated or SHH stimulated CD133 − and CD133 + R28 cells.
Article Snippet: For isolation of CD133 positive stem cells, the
Techniques: Comparison, Staining, Double Staining
Journal: Oncotarget
Article Title: Hedgehog signaling sensitizes Glioma stem cells to endogenous nano-irradiation
doi:
Figure Lengend Snippet: Representative fluorescent images after staining with anti-CD133 antibody (green; i, v), anti-SOX-2 antibody (red; ii, vi), anti-Nestin antibody (green; iii, vii) and anti-GFAP antibody (green; iv, viii) of unstimulated or SHH stimulated CD133 + R28 cells (Hoechst nuclei staining in blue). The neurospheres are marked by arrows.
Article Snippet: For isolation of CD133 positive stem cells, the
Techniques: Staining
Journal: Oncotarget
Article Title: Hedgehog signaling sensitizes Glioma stem cells to endogenous nano-irradiation
doi:
Figure Lengend Snippet: (A) Cellular uptake of [I-125]ITdU (% of incubated dose (ID)/well) in normal human astrocytes in dependency on FdUrd and SHH stimulation. Data are mean ± SD from three experiments. (B) Cellular uptake of [I-125]ITdU (% of incubated dose (ID)/well) in CD133 − and CD133 + R28 cells in dependency on FdUrd and SHH stimulation. Data are mean ± SD from three experiments. (C) SDS-PAGE and Western blot analysis of TS expression in CD133 − and CD133 + R28 cells with and without conditioning with FdUrd and SHH. GAPDH served as a loading control. (D) DNA incorporation of [I-125]ITdU (% DNA association relative to total cellular uptake) in CD133 − and CD133 + R28 cells in dependence on FdUrd and SHH stimulation. Data are mean ± SD from three experiments.
Article Snippet: For isolation of CD133 positive stem cells, the
Techniques: Incubation, SDS Page, Western Blot, Expressing
Journal: Oncotarget
Article Title: Hedgehog signaling sensitizes Glioma stem cells to endogenous nano-irradiation
doi:
Figure Lengend Snippet: (A) SDS-PAGE and Western blot analysis of the activation of checkpoint response, DNA repair mechanisms and induction of apoptosis in response to [I-125]ITdU with FdUrd w/o SHH in CD133 − and CD133 + R28 cells (left). SDS-PAGE and Western blot analysis of apoptosis marker in CD133 − and CD133 + R28 cells after treatment with FdUrd w/o SHH (right). GAPDH served as a loading control. (B) The percentage of CD133 − and CD133 + R28 viable cells after incubation with [I-125]ITdU in dependency on FdUrd and SHH stimulation. Data are mean ± SD from three experiments. (C) Clonogenic survival of CD133 − and CD133 + R28 cells after incubation with [I-125]ITdU in dependency on FdUrd and SHH stimulation. Data are mean ± SD from three experiments.
Article Snippet: For isolation of CD133 positive stem cells, the
Techniques: SDS Page, Western Blot, Activation Assay, Marker, Incubation